IgE Modulates Neutrophil Survival in Asthma: Role of Mitochondrial Pathway

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作者：

AS Saffar，MP Alphonse，L Shan，KT Hayglass，FER Simons，AS Gounni

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摘要：

The high-affinity IgE receptor (FcepsilonRI) has recently been reported to be expressed by neutrophils in atopic asthmatic individuals, leading to speculations that IgE could influence biological functions of these cells. In this study, we demonstrate that monomeric human IgE delayed spontaneous apoptosis of primary human neutrophils from atopic asthmatics in vitro. This effect was not dependent on FcepsilonRI cross-linking or autocrine release of soluble mediators; however, it was associated with increased expression of the antiapoptotic myeloid cell leukemia-1 protein, retention of the proapoptotic molecule Bax in the cytoplasm, decreased release of Smac from mitochondria, and reduced caspase-3 activity. Taken together, our results indicate that in vitro IgE can delay programmed cell death of neutrophils from allergic asthmatics and this may possibly contribute to neutrophilic inflammation in atopic asthma.

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关键词：

Neutrophils Cytoplasm Mitochondria Humans Asthma Inflammation Intracellular Signaling Peptides and Proteins Immunoglobulin E Receptors, IgE Mitochondrial Proteins