Myosin X is a downstream effector of PI(3)K during phagocytosis
摘要：
Phagocytosis is a phosphatidylinositol-3-OH-kinase (PI(3)K)-dependent process in macrophages. We identified Myo10 (Myosin-X), an unconventional myosin with pleckstrin homology (PH) domains, as a potential downstream target of PI(3)K. Myo10 was recruited to phagocytic cups in a wortmannin-sensitive manner. Expression of a truncation construct of Myo10 (Myo10 tail) in a macrophage cell line or cytosolic loading of anti-Myo10 antibodies in bovine alveolar macrophages inhibited phagocytosis. In contrast, expression of a Myo10 tail construct containing a point mutation in one of its PH domains failed to inhibit phagocytosis. Expression of Myo10 tail inhibited spreading, but not adhesion, on IgG-coated substrates, consistent with a function for Myo10 in pseudopod extension. We propose that Myo10 provides a molecular link between PI(3)K and pseudopod extension during phagocytosis.
展开
关键词：
Animals Cattle Mice Macrophages Cell Line Pseudopodia Myosins Androstadienes Microscopy, Fluorescence Point Mutation
DOI：
10.1038/sj.ejhg.5201573
被引量：
Nature
Springer
Nature
(全网免费下载)
EBSCO
万方医学
查看更多
dx.doi.org
NCBI
NCBI
Europe PMC
ResearchGate
(全网免费下载)
ResearchGate
ProQuest
EBSCO
EBSCO
EBSCO
molbiolcell.org
einstein.in
(全网免费下载)
embor.embopress.org
einstein.yu.edu
(全网免费下载)
frontiersin.org
www.socolar.com
mysciencework.com
pubfacts.com
pubget.com
physiolgenomics.physiology.org
f1000.com
coxcammer.com
(全网免费下载)
deepdyve.com
prodeinsteinwebsite.vipprod.dmz.yu.edu
(全网免费下载)
einstein.yu.edu
(全网免费下载)
jcs.biologists.org
Nature
(全网免费下载)
ResearchGate
(全网免费下载)
prodeinsteinwebsite.vipprod.dmz.yu.edu
(全网免费下载)
einstein.yu.edu
(全网免费下载)
einstein.in
(全网免费下载)
coxcammer.com
(全网免费下载)
einstein.yu.edu
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
