Production and physiological actions of anandamide in the vasculature of the rat kidney.
摘要：
The endogenous cannabinoid receptor agonist anandamide is present in central and peripheral tissues. As the kidney contains both the amidase that degrades anandamide and transcripts for anandamide receptors, we characterized the molecular components of the anandamide signaling system and the vascular effects of exogenous anandamide in the kidney. We show that anandamide is present in kidney homogenates, cultured renal endothelial cells (EC), and mesangial cells; these cells also contain anandamide amidase. Reverse-transcriptase PCR shows that EC contain transcripts for cannabinoid type 1 (CB1) receptors, while mesangial cells have mRNA for both CB1 and CB2 receptors. EC exhibit specific, high-affinity binding of anandamide (Kd = 27.4 nM). Anandamide (1 microM) vasodilates juxtamedullary afferent arterioles perfused in vitro; the vasodilation can be blocked by nitric oxide (NO) synthase inhibition with L-NAME (0.1 mM) or CB1 receptor antagonism with SR 141716A (1 microM), but not by indomethacin (10 microM). Anandamide (10 nM) stimulates CB1-receptor-mediated NO release from perfused renal arterial segments; a similar effect was seen in EC. Finally, anandamide (1 microM) produces a NO-mediated inhibition of KCl-stimulated [3H]norepinephrine release from sympathetic nerves on isolated renal arterial segments. Hence, an anandamide signaling system is present in the kidney, where it exerts significant vasorelaxant and neuromodulatory effects.
展开
关键词：
Kidney Sympathetic Nervous System Cells, Cultured Animals Rats Rats, Sprague-Dawley Nitric Oxide Ethanolamines Norepinephrine Cannabinoids
DOI：
10.1172/JCI119677
被引量：
年份：
1997
Semantic Scholar
(全网免费下载)
Semantic Scholar
万方医学
掌桥科研
dx.doi.org
查看更多
Europe PMC
(全网免费下载)
NCBI
Pubmed Central
(全网免费下载)
Oxford Univ Press
NCBI
Europe PMC
ResearchGate
ResearchGate
(全网免费下载)
ProQuest
EBSCO
Citeseer
(全网免费下载)
molpharm.aspetjournals.org
druglibrary.org
(全网免费下载)
cifas.us
(全网免费下载)
beckleyfoundation.org
(全网免费下载)
hyper.ahajournals.org
dm5migu4zj3pb.cloudfront.net
(全网免费下载)
jlr.org
proxychi.baremetal.com
(全网免费下载)
jcs.biologists.org
gastrojournal.org
druglibrary.net
(全网免费下载)
ajpheart.physiology.org
cifas.us
(全网免费下载)
jci.org
jimmunol.org
fasebj.org
jasn.asnjournals.org
学术范
学术范
(全网免费下载)
mysciencework.com
core.ac.uk
proxy.baremetal.com
(全网免费下载)
druglibrary.org
(全网免费下载)
scienceopen.com
static.jci.org
(全网免费下载)
SAGE
druglibrary.net
(全网免费下载)
jci.org
(全网免费下载)
Semantic Scholar
(全网免费下载)
Pubmed Central
(全网免费下载)
Europe PMC
(全网免费下载)
ResearchGate
(全网免费下载)
Citeseer
(全网免费下载)
cifas.us
(全网免费下载)
static.jci.org
(全网免费下载)
jci.org
(全网免费下载)
druglibrary.net
(全网免费下载)
druglibrary.org
(全网免费下载)
druglibrary.net
(全网免费下载)
proxy.baremetal.com
(全网免费下载)
beckleyfoundation.org
(全网免费下载)
cifas.us
(全网免费下载)
proxychi.baremetal.com
(全网免费下载)
druglibrary.org
(全网免费下载)
dm5migu4zj3pb.cloudfront.net
(全网免费下载)
学术范
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
