An enzymatic amplification cycle for high sensitive immunoassay
摘要：
An amplification cycle consisting of oligosaccharide dehydrogenase (ODH) and laccase is found to be highly sensitive to several catecholamines and p -aminophenol. An immunoassay is constructed by combining this amplification cycle with a suitable enzyme label, of which p -aminophenol is the product of the enzymatic reaction. Alkaline phosphatase and β-galactosidase were tested as labels for immunoglobulin G from goat (gtIgG) and human thyroid stimulating hormone (hTSH) immunoassay. The combination of enzymatic amplification cycles with a sandwich type immunoassay yields high sensitivity measurement of hTSH. The assay was found to have two linear ranges: the first one is between 0.2 and 20 ng/ml, the second one from 0.0005 to 0.5 ng/ml, depending on assay procedure. Both together cover well the diagnostically relevant range of 0.1–100 mU/l (0.017-17 ng/ml); the detection limit is at 0.3 pg/ml (1.8 μU/l).
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关键词：
immunoassay biochemical amplifier bienzyme electrode oligosaccharide dehydrogenase laccase thyroid stimulating hormone hTSH
DOI：
10.1016/0003-2670(96)00046-3
被引量：
年份：
1996
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