Directed Multistep Biocatalysis for the Synthesis of the Polyketide Oxytetracycline in Permeabilized Cells of Escherichia coli
摘要:
We report on tailored regeneration modules for the essential cofactors ATP and NADPH as well as for SAM supply, adapted to support biocatalytic multistep synthesis of oxytetracycline (OTC) in permeabilized cells of E. coli. ATP is regenerated from ADP and AMP by the use of polyphosphate. The efficiency of this system was proven with permeabilized cells producing flaviolin. NADPH is regenerated from NADP+ by oxidation of phosphonate. A constant level of 0.7 mg/L of SAM is provided by the ligation of ATP and methionine. OTC is synthesized at a rate of 2.05 ng/(h (g of the combined biocatalyst)), reaching a final concentration of 0.9 mg/L.
展开
关键词:
flaviolin oxytetracycline S-adenosylmethionine β-nicotinamide-adenine-dinucleotide-phosphate adenosine-5′-triphosphate permeabilized cells Escherichiacoli biocatalysis
DOI:
10.1021/cs501825u
年份:
2015
通过文献互助平台发起求助,成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
来源期刊
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用!