摘要：

A rat osteosarcoma cell clone (ROS 17/2), and osteoblast-enriched populations from rat calvaria cultured in the presence of concanavalin A, have been shown to produce latent collagenase and collagenase inhibitors. The enzymes and inhibitor activities from the ROS 17/2 cells were concentrated by ammonium sulphate precipitation and separated by gel filtration on AcA 54 resin. The size of the latent collagenase (Mr∼ 58000) was reduced on conversion to active enzyme (Mr∼ 48000) by p-aminophenylmercuric acetate. Latent and active forms of gelatinase activity, similar in size to the corresponding forms of collagenase, were also resolved. The collagenase inhibitor activity, which was sensitive to organomercurials, was recovered in two peaks (Mr∼ 68000 and 30000). The active collagenase cleaved interstitial collagens (type I = III > II) producing typical 3/4 and 1/4 fragments. This activity was inhibited by the metal ion chelators ethylenediamine

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