Rab5 regulates motility of early endosomes on microtubules
摘要：
The small GTPase Rab5 regulates membrane docking and fusion in the early endocytic pathway. Here we reveal a new role for Rab5 in the regulation of endosome interactions with the microtubule network. Using Rab5 fused to green fluorescent protein we show that Rab5-positive endosomes move on microtubules in vivo. In vitro, Rab5 stimulates both association of early endosomes with microtubules and early-endosome motility towards the minus ends of microtubules. Moreover, similarly to endosome membrane docking and fusion, Rab5-dependent endosome movement depends on the phosphatidylinositol-3-OH kinase hVPS34. Thus, Rab5 functionally links regulation of membrane transport, motility and intracellular distribution of early endosomes.
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关键词：
Humans Microtubules Endosomes Cell Line Hela Cells rab5 GTP-Binding Proteins Recombinant Fusion Proteins Luminescent Proteins Green Fluorescent Proteins Biological Transport
DOI：
10.1038/14075
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年份：
1999
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