Effect of dibutyryl cyclic adenosine monophosphate on granulosa cell proliferation, oocyte growth and meiotic maturation in isolated mouse primary ovarian follicles cultured in collagen gels.

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摘要：

Isolated primary follicles from 10-day-old were cultured in a gel matrix for 6 days in Minimum Essential Medium + foetal calf serum, followed by culture in unsupplemented medium (control) or in medium containing (2 mM) or dibutyryl cyclic (dbcAMP, 0.25 mM) for a further 3 or 6 days. Less than 10% of oocytes resumed meiosis during the culture period in all groups. At recovery, the diameter of oocytes at the stage was recorded and their ability to resume meiosis was determined. had little effect on and meiotic competence, but culture in dbcAMP resulted in oocytes that were larger (60.2 +/- 0.6 microns) than those of controls (55.8 +/- 0.5 microns) and more competent to resume meiosis than were controls (42.9% and 10.8%, respectively). The addition of dbcAMP to the culture medium induced a 4-5-fold increase in the number of granulosa oocyte compared with controls (3757 +/- 423 and 838 +/- 93, respectively). These results indicate that increased and meiotic competence is primarily mediated via dbcAMP effects on the granulosa .

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关键词：

PRIMARY FOLLICLE OOCYTE MEIOTIC MATURATION GRANULOSA CELL MOUSE

DOI：

10.1530/jrf.0.0920197

被引量：

141

年份：

1991