Contacting target nucleic acid with primer so primer anneals to nucleic acid in sequence specific manner, incorporating hydrocarbyldithiomethyl-modified nucleotide into primer, detecting modified nucleotide, identifying sequence of base
公开/公告号:
US6309836 B1
公开/公告日期:
2001年10月30日
被引量:
摘要:
or a salt thereof wherein R 1 is an organic molecule and R 2 is a hydrocarbyl is useful for protecting and/or blocking hydroxyl groups in organic molecules such as nucleotides. The hydrocarbyldithiomethyl-modified compounds can also be used for chemically synthesizing oligonucleotides and for sequencing nucleic acid compounds.
展开
通过文献互助平台发起求助,成功后即可免费获取论文全文。
请先登入
相似文献
参考文献
引证文献
研究点推荐
引用走势
2010
被引量:18
辅助模式
0
引用
文献可以批量引用啦~
欢迎点我试用!