Polyelectrolyte Multilayer Nanofilms Used as Thin Materials for Cell Mechano-Sensitivity Studies

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作者：

T Boudou，T Crouzier，C Nicolas，K Ren，C Picart

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摘要：

Three types of multilayer films made from poly(L-lysine)/hyaluronan, chitosan/hyaluronan, and poly(allylamine hydrochloride)/poly(L-glutamic acid), were used to investigate the interplay between film mechanoâchemical properties and cell adhesion. We showed that C2C12 myoblast adhesion and proliferation depended on the extent of film cross-linking for all films whatever their internal chemistry. Cell spreading areas were found to correlate with the film's stiffness and to be distributed over a unique curve. Immuno-staining of the cytoskeletal components revealed the formation of F-actin stress fibers and vinculin plaques only on stiff films. Finally, we compared our results with previous studies performed on polyacrylamide and PDMS gels, two recognized materials for mechano-sensitivity studies. We found that the effect of substrate stiffness on cell spreading is material-dependent.

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关键词：

cell adhesion and spreading cross-linking fibers layer-by-layer assembly mechanical properties mechano-sensitivity

DOI：

10.1002/mabi.201000301

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年份：

2011