The construction in vitro of transducing derivatives of phage lambda
摘要:
Methods are described for the construction of plaque-forming, transducing derivatives of phage lambda, using appropriate receptor genomes and fragments of DNA generated by the restriction enzymes endo R. Eco RI and endo R. Hind III. The general properties of the transducing derivatives are described and discussed. Plaque-forming phages carrying the E. coli trp, his, cysB, thyA, supD, supE, supF, hsd, tna and lig genes have been isolated.
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DOI:
10.1007/BF00268089
被引量:
年份:
1976
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