摘要：

The rat NMDAR1 (N-methyl-d-aspartate receptor) was expressed transiently in human embryonic kidney cells. Transfected cell homogenates showed saturable [³H]MK-801 binding activity that was best fit by a single high-affinity site with a K_D of 9 nM and a 5_max of 113 fmol of binding sites/mg of protein. Antibodies raised against the peptide sequence NMDAR1 (929–938) coupled to keyhole limpet haemocyanin specifically recognised a single band with M_r 117,000 in immunoblots from adult rat brain. In the transfected cells, the antibody recognised two bands: one with M_r 117,000, which was coincident with that from brain membranes, and one with M_r 97,000, which was identified as nonglycosylated NMDAR1 subunit. These results identify the NMDAR1 of rat brain and further show that the homooligomer binds MK-801, albeit at low efficiency.

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