DNA METHYLATION ANALYSIS BY DIGITAL BISULFITE GENOMIC SEQUENCING AND DIGITAL METHYLIGHT
摘要：
Provided are novel sensitive methylation assays referred to herein as Digital MethyLight, comprising stochastically distributing and compartmentalizing bisulfite-treated genomic DNA over multiple PCR reaction wells for detection of individually methylated DNA molecules in a large background of unmethylated DNA. Digital Bisulfite Genomic DNA Sequencing methods are also provided for high-resolution DNA methylation information without subcloning. Background signal and PCR contaminants are diluted, while the ratio of primer to methylated template DNA is kept high. Preferably, biological fluid (e.g., urine, blood-based (e.g., plasma and/or serum)) samples are analyzed for cancer diagnosis, prognosis and surveillance. Multiplexed PCR formats may be implemented to enhance when using small DNA amounts. Compositions and methods for diagnosis and/or prognosis of breast cancer, comprising the use of FOXE1, CLDN5 and/or RUNX3 gene markers are also provided (SEQ ID NOS: 17, 16 and 18, respectively for respective CpG island sequences), and in preferred embodiments plasma or serum samples are used.
展开
关键词：
Humans Breast Neoplasms Sulfites DNA, Neoplasm Polymerase Chain Reaction CpG Islands Genomics Microfluidic Analytical Techniques DNA Methylation
DOI：
10.1093/nar/gkn455
被引量：
年份：
2008
国家科技图书文献中心
(权威机构)
EBSCO
万方
academic.oup.com
掌桥科研
查看更多
Semantic Scholar
(全网免费下载)
Semantic Scholar
万方医学
dx.doi.org
Europe PMC
(全网免费下载)
NCBI
Europe PMC
Oxford Univ Press
Oxford Univ Press
(全网免费下载)
NCBI
(全网免费下载)
NCBI
AGRIS
FreePatentsOnline
ProQuest
ResearchGate
(全网免费下载)
ResearchGate
EBSCO
Citeseer
(全网免费下载)
Citeseer
EBSCO
gene-quantification.net
(全网免费下载)
paperity.org
Oxford University Press (OUP)
(全网免费下载)
gmo-qpcr-analysis.com
(全网免费下载)
gmo-qpcr-analysis.info
(全网免费下载)
gene-quantification.biz
(全网免费下载)
biomedsearch.com
(全网免费下载)
qpcrupdate.net
(全网免费下载)
gene-quantification.org
(全网免费下载)
science-open.com
(全网免费下载)
gmo-qpcr-analysis.info
(全网免费下载)
gene-quantification.com
(全网免费下载)
mysciencework.com
gene-quantification.de
(全网免费下载)
qpcrupdate.eu
(全网免费下载)
gene-quantification.biz
(全网免费下载)
学术范
(全网免费下载)
gene-quantification.org
(全网免费下载)
gmo-qpcr-analysis.com
(全网免费下载)
gene-quantification.net
(全网免费下载)
cebp.aacrjournals.org
学术范
qpcrupdate.org
(全网免费下载)
qpcrupdate.info
(全网免费下载)
gene-quantification.com
(全网免费下载)
clinchem.org
qpcrupdate.info
(全网免费下载)
scienceopen.com
science-open.com
gene-quantification.eu
(全网免费下载)
readcube.com
mendeley.com
invenzone.com
gene-quantification.eu
(全网免费下载)
deepdyve.com
pubfacts.com
qpcrupdate.org
(全网免费下载)
pherobase.com
qpcrupdate.com
(全网免费下载)
bvsalud.org
qpcrupdate.net
(全网免费下载)
gene-quantification.de
(全网免费下载)
Google Patents
Semantic Scholar
(全网免费下载)
NCBI
(全网免费下载)
Oxford Univ Press
(全网免费下载)
Europe PMC
(全网免费下载)
ResearchGate
(全网免费下载)
Citeseer
(全网免费下载)
gene-quantification.net
(全网免费下载)
qpcrupdate.org
(全网免费下载)
biomedsearch.com
(全网免费下载)
qpcrupdate.net
(全网免费下载)
gmo-qpcr-analysis.com
(全网免费下载)
science-open.com
(全网免费下载)
gene-quantification.de
(全网免费下载)
gmo-qpcr-analysis.com
(全网免费下载)
qpcrupdate.info
(全网免费下载)
qpcrupdate.info
(全网免费下载)
qpcrupdate.net
(全网免费下载)
gene-quantification.eu
(全网免费下载)
Oxford University Press (OUP)
(全网免费下载)
gene-quantification.biz
(全网免费下载)
gmo-qpcr-analysis.info
(全网免费下载)
qpcrupdate.org
(全网免费下载)
gene-quantification.eu
(全网免费下载)
gene-quantification.com
(全网免费下载)
qpcrupdate.eu
(全网免费下载)
gene-quantification.org
(全网免费下载)
qpcrupdate.com
(全网免费下载)
gmo-qpcr-analysis.info
(全网免费下载)
gene-quantification.org
(全网免费下载)
gene-quantification.net
(全网免费下载)
gene-quantification.biz
(全网免费下载)
gene-quantification.com
(全网免费下载)
学术范
(全网免费下载)
gene-quantification.de
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
来源期刊
引用走势
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
