Urinary d-lactate levels reflect renal function in aristolochic acid-induced nephropathy in mice

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作者：

Huang, Tzu-Chuan，Chen, Shih-Ming，Li, Yi-Chieh，Lee, Jen-Ai

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摘要：

Urinary d-lactate is highly correlated to diabetic nephropathy a progressive kidney disease in renal glomeruli. In this study, we used a C3H/3e mouse model to investigate the relationship between urinary d-lactate and aristolochic acid nephropathy where the glomerular structure is not affected. The nephropathy was induced using intravenous injections of aristolochic acid at a dosage of 10mg/kg per day for 5days and was characterized biochemically and histologically. The urinary excretions of proteins, N-acetyl--d-glucosaminidase and serum creatinine were determined and connected to histological conventional findings. Urinary d-lactate was analyzed using column-switching high-performance liquid chromatography with fluorescence detection. The results showed a remarkable increase of urinary markers, including of urinary proteins and N-acetyl--d-glucosaminidase, and the histological examination confirmed a diagnosis of acute tubule necrosis. The ratio of d-lactate to creatinine in the urine of aristolochic acid-treated mice was approximately 36 times greater than that of the mice in the control group (p<0.05). The ratios for the two groups of mice were 311.00±71.70 and 8.60±1.80mol/mmol creatinine, respectively. These data confirm in vivo that urinary d-lactate reflects renal injury conditions in aristolochic acid-treated mice and may be a marker for the assessment of nephropathy. Copyright 2013 John Wiley & Sons, Ltd.

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关键词：

aristolochic acid nephropathy renal injury d-lactate l-lactate urine

DOI：

10.1002/bmc.2908

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年份：

2013