Transition metal carbonyl oestrogen receptor assay
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18
摘要：
Hormonal steroids, such as 17 beta -estradiol derivatives, labeled with organometallic moieties have been synthesized and tested as markers for hormone receptor assay. Among the organometallic labels used were: Cr(CO)//3 and Cr(CO)//2 2 which can be attached to the arene A ring of the steroid; Co//2(CO)//6 and 2 (CO)//Mo( eta **5-C//5H//5)//2(CO)//4 which can be bonded to acetylenic groups introduced at the 17 alpha -position of the five-membered steroidal ring. There is a spectral window at about 2000 cm** minus **1 in the FT-IR spectra of the protein precipitated from lamb uterus cytosol which we reasoned could be used to detect the nu (CO) modes of the metal carbonyl fragments of the organometallic labels. This indeed proved possible and the present detection level for 10,000-20,000 co-added scans at 4 cm** minus **1 resolution is in the neighborhood of 100 fM.
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DOI：
10.1351/pac198557121865
被引量：
年份：
1985
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