SNAREpins: minimal machinery for membrane fusion.
摘要：
Recombinant v- and t-SNARE proteins reconstituted into separate lipid bilayer vesicles assemble into SNAREpins-SNARE complexes linking two membranes. This leads to spontaneous fusion of the docked membranes at physiological temperature. Docked unfused intermediates can accumulate at lower temperatures and can fuse when brought to physiological temperature. A supply of unassembled v- and t-SNAREs is needed for these intermediates to form, but not for the fusion that follows. These data imply that SNAREpins are the minimal machinery for cellular membrane fusion.
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关键词：
Cell Membrane Intracellular Membranes Escherichia coli Lipid Bilayers Bacterial Proteins Membrane Proteins Vesicular Transport Proteins Nerve Tissue Proteins Recombinant Proteins DNA, Bacterial
DOI：
10.1016/S0092-8674(00)81404-X
被引量：
年份：
1998
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