Isolation and characterization of an anaerobic ruminal bacterium capable of degrading hydrolyzable tannins.

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作者：

KE Nelson，AN Pell，P Schofield，S. H. Zinder

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摘要：

An anaerobic diplococcoid bacterium able to degrade hydrolyzable tannins was isolated from the ruminal fluid of a goat fed desmodium (Desmodium ovalifolium), a tropical legume which contains levels as high as 17% condensed tannins. This strain grew under anaerobic conditions in the presence of up to 30 g of tannic acid per liter and tolerated a range of phenolic monomers, including gallic, ferulic, and p-coumaric acids. The predominant fermentation product from tannic acid breakdown was pyrogallol, as detected by high-performance liquid chromatography and mass spectrometry. Tannic acid degradation was dependent on the presence of a sugar such as glucose, fructose, arabinose, sucrose, galactose, cellobiose, or soluble starch as an added carbon and energy source. The strain also demonstrated resistance to condensed tannins up to a level of 4 g/liter.

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关键词：

Cicer arietinum Fusarium oxysporum in vitro selection phenols enzymes

DOI：

10.1007/BF03321951

被引量：

193

年份：

1995