Conversion by Peyer's Patch Lymphocytes of Human Enterocytes into M Cells that Transport Bacteria

来自 Semantic Scholar

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作者：

S Kerneis，A Bogdanova，JP Kraehenbuhl，E Pringault

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摘要：

The epithelium that lines the gut is impermeable to macromolecules and microorganisms, except in Peyer's patches (PPs), where the lymphoid follicle-associated epithelium (FAE) contains M cells that transport antigens and microorganisms. A cultured system that reproduces the main characteristics of FAE and M cells was established by cultivation of PP lymphocytes with the differentiated human intestinal cell line Caco-2. Lymphocytes settled into the epithelial monolayer, inducing reorganization of the brush border and a temperature-dependent transport of particles and Vibrio cholerae. This model system could prove useful for intestinal physiology, vaccine research, and drug delivery studies.

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关键词：

Animals B-Lymphocytes immunology Bacterial Translocation Caco-2 Cells Carrier Proteins analysis Cell Polarity Coculture Techniques Fluorescein-5-isothiocyanate Humans Immunity Mucosal Intestinal Mucosa cytology immunology microbiology Lymphocytes immunology Mice Mice Inbred BALB C Microfilament Proteins analysis Microspheres Microvilli chemistry ultrastructure Peyer's Patches cytology immunology Research Support Non-U.S. Gov't Sucrase-Isomaltase Complex analysis T-Lymphocytes immunology Temperature Vibrio cholerae metabolism