Chromatography of 32P‐Labelled Oligonucleotides on Thin Layers of DEAE‐Cellulose
摘要：
A two-dimensional fractionation procedure has been developed for separating radioactively-labelled oligonucleotides of up to 50 residues long, using uniformly 32 P-labelled 5S RNA of Escherichia coli as a model compound. The method uses ionophoresis on cellulose acetate at pH 3.5 in the first dimension; and ascending chromatography with a concentrated mixture of oligonucleotides on thin layers of mixed DEAE-cellulose and cellulose in the second dimension.
展开
DOI：
10.1111/j.1432-1033.1969.tb00786.x
被引量：
年份：
1969
通过文献互助平台发起求助，成功后即可免费获取论文全文。
请先登入
相似文献
参考文献
引证文献
辅助模式
0
引用
文献可以批量引用啦~
欢迎点我试用！
