Order and intracellular location of the events involved in the maturation of a spliced tRNA
摘要：
Microinjected frog oocytes were used to analyse the RNA processing steps which lead to the appearance of a mature cytoplasmic tRNA tyr molecule. The results show that removal of the intervening sequence from within a yeast tRNA tyr precursor, excision of extra 3′ and 5′ nucleotides, addition of a 3′-terminal CCA and modification of at least seven ribonucleotides all occur in the nucleus before the tRNA tyr is transported to the cytoplasm. Moreover, we find that the ribonucleotide modifications occur in a strict order which precisely correlates with the size alterations of the tRNA tyr precursor.
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DOI：
10.1038/284143a0
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年份：
1980
Nature
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