Tunicamycin inhibition of polyisoprenyl-N-acetylglucosamine pyrophosphate formation in calf liver microsomes
摘要：
Calf-liver rough microsomes incorporated N-acetyl[ 3H] glucosamine from UDP-[ 3H]GlcNAc or [ 14C]mannose from GDP-[ 14C]Man into products soluble in chloroform-methanol (2:1). The [ 3H] GlcNAclabeled material had the hydrolytic and chromatographic properties of a polyisoprenyl N-acetylglucosaminyl pyrophosphate whereas the 14C-mannolipid exhibited the characteristics of a polyisoprenyl mannosyl phosphate. Tunicamycin inhibited the production of the [ 3H]G1cNAc-lipid but did not affect the synthesis of the 14C-mannolipid. This inhibition accounts for the effect of the antibiotic upon glycoprotein formation in vivo since polyprenyl sugar phosphates are intermediates in the biosynthesis of certain glycoproteins.
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关键词：
TM tunicamycin tunicamycin UDP uridine diphosphate uridine diphosphate GDP guanosine diphosphate guanosine diphosphate GlcNAc N-acetylglucosamine N-acetylglucosamine Man mannose mannose
DOI：
10.1016/S0006-291X(75)80086-6
被引量：
年份：
1975
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