Macromolecular Components of Tomato Fruit Pectin

来自 NCBI

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作者：

ML Fishman，KC Gross，DT Gillespie，SM Sondey

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摘要：

Chelate and alkaline-soluble pectin extracted from cell walls of pericarp tissue from mature green, turning, and red ripe ( Lycopersicon esculentum Mill.) fruit (cv. Rutgers), were studied by high-performance size-exclusion chromatography. Computer-aided curve fitting of the chromatograms to a series of Gaussian-shaped components revealed that pectin from all fractions was composed of a linear combination of five macromolecular-sized species. The relative sizes of these macromolecules as obtained from their radii of gyration were 1:2:4:8:16. Dialysis against 0.05 m NaCl induced partial dissociation of the biopolymers. Apparently, the weight fraction of smaller sized species increased at the expense of larger ones. Also, the dissociation produced low-molecular-weight fragments. Behavior in the presence of 0.05 m NaCl led to the conclusion that cell wall pectin acted as if it were an aggregated mosaic, held together at least partially through noncovalent interactions.

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关键词：

LGIC SUPERFAMILY PROTO-BINDING SITE CYS-LOOP MOTIF DOCKING MODEL ANIONIC SITE SPECIFICITY RESIDUE

DOI：

10.1016/0003-9861(89)90429-3

被引量：

136

年份：

1989