Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin.
摘要：
Discusses fluorescent indicators for Ca2+ based on green fluorescent proteins (GFP)and calmodulin. The difficulty in measuring important Ca2+ signals in the cytosol and organelles; The increase in the flourescence resonance energy transfer (FRET) between the flanking GFPs because of the binding of Ca2+ which makes calmodulin wrap around the M13 domain; FRET as an indicator of the reversible intermolecular association of cyan-GFP-labelled calmodulin with yellow-GFP-labelled M13.
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1997
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2010
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