One binding site determines sequence specificity of Tetrahymena pre-rRNA self-splicing, trans-splicing, and RNA enzyme activity.

阅读量：

作者：

展开

摘要：

The specificity of reactions catalyzed by the Tetrahymena pre-rRNA intervening sequence (IVS) was studied using site-specific mutagenesis. Two sequences required for 5′ splice-site selection during self-splicing were defined. Single-base changes in either a 5′ exon sequence or a 5′ exon-binding site within the IVS disrupt their ability to pair and result in inefficient or inaccurate splicing. Combinations that restore complementarity suppress the effect of the single-base changes. Sequence alterations in the 5′ exonbinding site also change the specificity of two other reactions: intermolecular exon ligation ( trans-splicing) and the enzymatic nucleotidyltransferase activity of the IVS RNA. Thus the substrate specificity of an RNA enzyme can be changed in a manner predictable by the rules of Watson-Crick base-pairing.

展开

关键词：

Nucleotidyltransferases RNA, Ribosomal Base Sequence Nucleic Acid Conformation Substrate Specificity Mutation Introns Exons Templates, Genetic RNA Splicing