Characterization of the blood–brain barrier choline transporter using the in situ rat brain perfusion technique
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21
摘要：
Choline enters brain by saturable transport at the blood–brain barrier (BBB). In separate studies, both sodium-dependent and passive choline transport systems of differing affinity have been reported at brain capillary endothelial cells. In the present study, we re-examined brain choline uptake using the in situ rat brain perfusion technique. Saturable brain choline uptake from perfusion fluid was best described by a model with a single transporter (Vmax= 2.4–3.1 nmol/min/g; Km = 39–42 µm) with an apparent affinity (1/Km) for choline five to ten-fold greater than previously reported in vivo, but less than neuronal ‘high-affinity’ brain choline transport (Km = 1–5 µm). BBB choline uptake from a sodium-free perfusion fluid using sucrose for osmotic balance was 50% greater than in the presence of sodium suggesting that sodium is not required for transport. Hemicho
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DOI：
10.1046/j.1471-4159.2001.00093.x
被引量：
年份：
2001
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