Generation of superoxide anion by the NADH dehydrogenase of bovine heart mitochondria
摘要：
Submitochondrial particles from bovine heart in which NADH dehydrogenase is reduced by either addition of NADH and rotenone or by reversed electron transfer generate 0.9 +/- 0.1 nmol of O2-/min per mg of protein at pH 7.4 and at 30 degrees C. When NADH is used as substrate, rotenone, antimycin and cyanide increase O2- production. In NADH- and antimycin-supplemented submitochondrial particles, rotenone has a biphasic effect: it increases O2- production at the NADH dehydrogenase and it inhibits O2- production at the ubiquinone-cytochrome b site. The generation of O2- by the rotenone, the uncoupler carbonyl cyanide rho-trifluoromethoxyphenylhydrazone and oligomycin at concentrations similar to those required to inhibit energy-dependent succinate-NAD reductase. Cyanide did not affect O2- generation at the NADH dehydrogenase, but inhibited O2- production at the ubiquinone-cytochrome b site. Production of O2- at the NADH dehydrogenase is about 50% of the O2- generation but the ubiquinone-cytochrome b area at pH 7.4. Additivity of the two mitochondrial sites of O2- generation was observed over the pH range from 7.0 to 8.8. AN O2- -dependent autocatalytic process that requires NADH, submitochondrial particles and adrenaline is described.
展开
关键词：
Animals Cattle Submitochondrial Particles Cyanides Superoxides Oxygen Antimycin A Rotenone Cytochrome Reductases NADH Dehydrogenase
DOI：
10.1042/bj1910421
被引量：
年份：
1980
BMJ
Springer
doi.org
掌桥科研
Semantic Scholar
(全网免费下载)
查看更多
万方医学
Oxford Univ Press
Europe PMC
NCBI
(全网免费下载)
NCBI
NCBI
NRC Research Press
AACR
JBC
ResearchGate
ResearchGate
(全网免费下载)
EBSCO
biochemj.org
(全网免费下载)
onAcademic
molbiolcell.org
mysciencework.com
genesdev.cshlp.org
scienceopen.com
reproduction-online.org
stroke.ahajournals.org
cheric.org
aggjournal.com
core.ac.uk
jeb.biologists.org
mcponline.org
molpharm.aspetjournals.org
jcb.rupress.org
garfield.library.upenn.edu
rstb.royalsocietypublishing.org
ajpcell.physiology.org
scindeks.ceon.rs
jacionline.org
emboj.embopress.org
cghjournal.org
diabetes.diabetesjournals.org
mendeley.com
gastrojournal.org
jneurosci.org
biochemj.org
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
来源期刊
引用走势
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
