摘要：

AIM: To investigate and explore the mechanism of apoptosis induced by Survivin antisense oligodeoxynucleotides (ASODN) that blocked out the expression of Survivin in pancreatic carcinoma cell line SW1990. METHODS: Survivin mRNA ASODN were designed and transfected into SW1990 cells by Lipofectimine. Apoptosis were measured using electron microscope (ECM) for morphology and flow cytometry (FCM) analysis with Annexin-V /PI dual staining for apoptotic cell ratio, caspase-3 and p38MAPK activity were measured using colorimetric assay and immuno-precipitation respectively. RESULTS: The predominant death model of SW1990 cells transfered Survivin mRNA ASODN was apoptosis. The apoptotic ratio in this group was increased significantly than that of control, SODN control and MMODN control groups after survivin ASODN transfection (55.3% vs 2.9%, 3.2%, 4.5%, and 4.8% respectively, P0.05). The activity of p38MAPK and caspase-3 were increased significantly after survivin ASODN transfection (8.81 ± 1.33 vs 47.87 ± 2.91, and 14.73 ± 1.36 vs 23.47 ± 3.61,P0.05). CONCLUSION: Survivin plays an important role in apoptosis of pancreatic cancer. Survivin mRNA ASODN transfered with lipofectimine can effectively activate p38MAPK-caspase-3 signal pathway sequentially and induce SW1990 cell apoptosis

展开