Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity.
摘要：
The RNA-guided Cas9 nuclease can tolerate certain mismatches to the DNA target and can thereby promote undesired off-target mutagenesis. A "double nicking" strategy has been developed to significantly reduce off-target activity in cell lines and to facilitate efficient gene knockout in mouse zygotes, enabling genome editing applications with higher levels of specificity.
展开
关键词：
ZINC-FINGER NUCLEASES TAL EFFECTORS HUMAN-CELLS BACTERIA SYSTEM TRANSCRIPTION ENDONUCLEASE GENERATION MUTATIONS KNOCKOUT
DOI：
10.1016/j.cell.2013.08.021
被引量：
年份：
2013
Elsevier
Elsevier
(全网免费下载)
Taylor & Francis
掌桥科研
万方医学
查看更多
万方医学
Semantic Scholar
(全网免费下载)
dx.doi.org
Ingenta
NCBI
(全网免费下载)
Europe PMC
(全网免费下载)
Europe PMC
NCBI
Cell Press
(全网免费下载)
www.cell.com
ResearchGate
(全网免费下载)
ResearchGate
Citeseer
(全网免费下载)
EBSCO
ozyme.fr
(全网免费下载)
mysciencework.com
zhanglab.tch.harvard.edu
(全网免费下载)
advances.sciencemag.org
plantphysiol.org
knockout.cwru.edu
(全网免费下载)
core.ac.uk
(全网免费下载)
science-open.com
onAcademic
idi.harvard.edu
horizondiscovery.com
(全网免费下载)
ko2.cwru.edu
(全网免费下载)
zlab.mit.edu
(全网免费下载)
f1000.com
msb.embopress.org
cancerletters.info
emergtoplifesci.org
genome.cshlp.org
ajkd.org
infona.pl
ko.case.edu
(全网免费下载)
stacks.cdc.gov
stacks.cdc.gov
(全网免费下载)
118.145.16.217
yadda.icm.edu.pl
genetics.org
mendeley.com
pubfacts.com
scienceopen.com
bio.biologists.org
hsu.salk.edu
(全网免费下载)
hdl.handle.net
jove.com
Elsevier
(全网免费下载)
Semantic Scholar
(全网免费下载)
Europe PMC
(全网免费下载)
NCBI
(全网免费下载)
Cell Press
(全网免费下载)
ResearchGate
(全网免费下载)
Citeseer
(全网免费下载)
zhanglab.tch.harvard.edu
(全网免费下载)
horizondiscovery.com
(全网免费下载)
stacks.cdc.gov
(全网免费下载)
zlab.mit.edu
(全网免费下载)
core.ac.uk
(全网免费下载)
ozyme.fr
(全网免费下载)
knockout.cwru.edu
(全网免费下载)
ko.case.edu
(全网免费下载)
ko2.cwru.edu
(全网免费下载)
hsu.salk.edu
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
