摘要：

We describe a simple and efficient method for the recovery of DNA fragments from agarose or acrylamide gels. The procedure involves electrophoresis of the fragments onto strips of DEAE-cellulose paper inserted in the gel between bands visualized by ethidium bromide fluorescence. The electrophoretic transfer is achieved in the original resolving gel using the same apparatus, thereby enabling purification of DNA fragments up to 20 kb in size with a yield of 60–80%. DNA which has been recovered in this way can be used for restriction enzyme cleavage, nick translation, end labeling by T 4 polynucleotide kinase, DNA sequencing, and electron microscopy. The DNA fragments can be recloned in pBR322 using either blunt-end or cohesive-end ligation. Thus our method is useful for the recovery of biologically active DNA from both agarose and high-percentage polyacrylamide gels.

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