High efficiency optical system detecting light from e.g. excited marked biomolecules
申请(专利)号：
DE19810615A
申请日期：
03/12/1998
公开/公告号：
DE19810615
公开/公告日期：
09/16/1999
申请(专利权)人：
Thomas Ruckstuhl , Stefan Seeger
被引量：
摘要：
One plane end face of an optical element (1) faces the sample, interfacing with the sample medium, or with an intervening medium of higher optical density. All light emitted from the sample not deflected by total internal reflection, enters the optical element, including transiently radiated light. Once inside the element, all light is reflected through the second end face (4) onto a detector (30). The excitation light beam is seen to pass axially through the equipment, to be trapped by the absorber (27). Scattered and evanescent light (7) undergoes total internal reflection at the paraboloidal walls of the optical element, before being focused (29) onto the detector (30). The sample is situated at the focus of the element, a paraboloidal reflector, an arrangement capturing a maximum of emitted light.
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