Identification and Characterization of an RNA-Dependent RNA Polymerase Activity within the Nonstructural Protein 5B Region of Bovine Viral Diarrhea Virus
摘要：
Nonstructural protein 5B (NS5B) of bovine viral diarrhoea virus (BVDV) contains sequence motifs that are predictive of an RNA-dependent RNA polymerase activity. The expression and purification of the BVDV NS5B protein derived from an infectious cDNAclone of BVDV (NADL strain) are described. BVDV NS5B protein was active in an in vitro RNA polymerase assay using homopolymeric RNA or BVDV minigenomic RNA templates. The major product was a covalently linked double-stranded molecule generated by a "copy-back" mechanism from the input template RNA. A nucleotide-nonspecific and template-independent terminal nucleotidyl transferase activity was also detected with the BVDV NS5B preparation.
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DOI：
10.1128/JVI.72.11.9365-9369.1998
被引量：
年份：
1998
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