Splicing of yeast tRNA precursors: structure of the reaction intermediates
摘要：
The intermediates of the yeast tRNA splicing reaction have been characterized. The intervening sequence is excised as an unique linear molecule. It has 5′-hydroxyl and 3′-phosphate termini. Correspondingly, the half-tRNA molecules are shown to have a 3′-phosphate terminus on the 5′ half and 5′-hydroxyl terminus on the 3′ half. These isolated halves have been shown to be active in the ligation step of tRNA splicing. Removal of the 3′-phosphate from the 5′ half eliminates the ability of the 5′ half to participate in ligation.
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关键词：
Saccharomyces cerevisiae Phosphates RNA, Transfer, Amino Acyl RNA, Fungal Base Sequence Nucleic Acid Conformation Nucleic Acid Precursors
DOI：
10.1016/0092-8674(79)90351-9
被引量：
年份：
1979
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