Biological Significance of Unwinding Capability of Nuclear Matrix-Associating DNAs
摘要：
Matrix attachment regions (MARs) are thought to separate chromatin into topologically constrained loop domains. A MAR located 5′ of the human β-interferon gene becomes stably base-unpaired under superhelical strain, as do the MARs flanking the immunoglobulin heavy chain gene enhancer; in both cases a nucleation site exists for DNA unwinding. Concatemerized oligonucleotides containing the unwinding nucleation site exhibited a strong affinity for the nuclear scaffold and augmented SV40 promoter activity in stable transformants. Mutated concatemerized oligonucleotides resisted unwinding, showed weak affinity for the nuclear scaffold, and did not enhance promoter activity. These results suggest that the DNA feature capable of relieving superhelical strain is important for MAR functions.
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关键词：
Humans Nuclear Matrix Hydrazines Oligodeoxyribonucleotides DNA Interferon-beta Electrophoresis, Polyacrylamide Gel Restriction Mapping Base Sequence Plasmids
DOI：
10.1126/science.1553545
被引量：
年份：
1992
万方医学
dx.doi.org
NCBI
Oxford Univ Press
NCBI
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