INDUCTION OF STREPTOMYCIN RESISTANCE IN SENSITIVE HEMOPHILUS INFLUENZAE BY EXTRACTS CONTAINING DESOXYRIBONUCLEIC ACID FROM RESISTANT HEMOPHILUS INFLUENZAE

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摘要：

Resistance to streptomycin, of a degree exceeding 1000 g. per ml., has been induced in sensitive strains ofHemophilus influenzaeby exposure for 10 minutes to desoxyribonucleic acid-containing extracts isolated from a strain of type bHemophilus influenzaewhich had emerged resistant to 1000 g. of streptomycin per ml. DNA is essential for the process which brings out this change; the reaction can be prevented by destruction of the DNA with crystalline desoxyribonuclease.The resistant trait which is created in this way is heritable. The nature of the process which induces resistance is similar in all respects to the reaction which induces heritable changes in type specificity ofH. influenzae. These results offer another example of the gene-like action of highly specific DNA's. The pattern of resistance brought out in a bacterial population exposed to the DNA-containing, resistance-inducing extract, is similar to that which occurs when emergence of resistance ofH. influenzaeto streptomycin follows the selection by streptomycin of spontaneously occurring resistant mutants. The change in a bacterial cell from average susceptibility to streptomycin to resistance to 1000 g. of streptomycin per ml. can occur in a single step.

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关键词：

antibiotics RNA streptomycin

DOI：

10.1084/jem.97.1.17

被引量：

161

年份：

1953