Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry

Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometryPeptidesMolecular analysis of complex biological structures and ...

M Wilm，T Houthaeve，S Breit，... - 《Nature》

FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex.

we utilized nano-electrospray tandem mass spectrometry, a recently developed technique to sequence femtomole quantities of polyacrylamide gel-separated proteins. ...

M Muzio，AM Chinnaiyan，FC Kischkel，... - 《Cell》

A generic strategy to analyze the spatial organization of multi-protein complexes by cross-linking and mass spectrometry.

Most cellular functions are performed by multi-protein complexes. The identity of the members of such complexes can now be determined by mass spectrometry....

J Rappsilber，S Siniossoglou，EC Hurt，... - 《Analytical Chemistry》

In vitro assembly of the mouse U14 snoRNP core complex and identification of a 65-kDa box C/D-binding protein.

(1996). Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry. Nature 379, 466-469.Watkins, N.J., Newman, D....

NJ Watkins，DR Newman，JF Kuhn，... - RNA

A high-performance liquid chromatography based strategy for rapid, sensitive sequencing of N-linked oligosaccharide modifications to proteins in sodium dod...

The majority of biologically active proteins are glycosylated, therefore any approach to proteomics which fails to address the analysis of oligosaccharides...

Pauline M. Rudd，C Colominas，L Royle，... - 《Proteomics》

Preparing a metal-ion chelated immobilized enzyme reactor based on the polyacrylamide monolith grafted with polyethylenimine for a facile regeneration and ...

Initially, a poly (glycidyl methacrylate-co-acrylamide-co-methylenebisacrylamide) monolith was prepared in the 100 渭m i.d. capillary, and then was grafted...

S Wu，L Zhang，K Yang，... - 《Analytical & Bioanalytical Chemistry》

A peptide concentration and purification method for protein characterization in the subpicomole range using matrix assisted laser desorption/ionization‐po...

this concentration procedure in combination with MALDI-post-source decay analysis to identify subpicomole amounts of proteins present in polyacrylamide gels. ...

K Gevaert，H Demol，T Sklyarova，... - 《Electrophoresis》

The tools of proteomics.

The chapter focuses on the tools used in proteomics. Mass spectrometry (MS)-based methods have become the primary technology used to identify proteins. The...

JA Loo - 《Advances in Protein Chemistry》

Developments and Applications of Electrophoresis and Small Molecule Laser Desorption Ionization Mass Spectrometry

Ultra-sensitive native fluorescence detection of proteins with miniaturized one- and two-dimensional polyacrylamide gel electrophoresis was achieved with l...

Z Hui - 《Office of Scientific & Technical Information Technical Reports》