Autoradiographic Visualization of A1 Adenosine Receptors in Rat Brain with [3H]8‐Cyclopentyl‐1,3‐Dipropylxanthine
摘要:
A 1 adenosine receptors were labeled in rat brain sections with the antagonist [ 3 H]8-cyclopentyl-1,3-dipropylxanthine ([ 3 H]DPCPX) and visualized at the light microscopic level using autoradiography. The specific binding of [ 3 H]DPCPX to the sections showed the pharmacological characteristics of A 1 adenosine receptors and was accompanied by very low levels of nonspecific binding. Whereas GTP had no significant effect on [ 3 H]DPCPX binding to rat brain membranes, the addition of 100 μ M GTP increased the apparent affinity of [ 3 H]DPCPX to tissue sections fivefold (from 1.83 to 0.35 n M ), enhancing it to the affinity measured in membranes. However, GTP altered neither the binding capacity nor the distribution of binding sites in tissue sections. It is suggested that a competitive antagonism with endogenous adenosine explains the lower affinity of [ 3 H]DPCPX in the absence of GTP. The autoradiographic pattern of [ 3 H]DPCPX binding was characteristic for A 1 adenosine receptors. Distinct labeling of the different layers of the cerebellar cortex was shown by photomicrographs generated with the coverslip technique. In addition, several fiber tracts were found to be labeled. The high selectivity for A 1 adenosine receptors and low nonspecific binding of [ 3 H]DPCPX, the ability to produce high-resolution autoradiograms, together with the fact that the effects of endogenous adenosine can be eliminated by the addition of GTP make [ 3 H]DPCPX a very useful tool in the autoradiographic study of A 1 adenosine receptors.
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DOI:
10.1111/j.1471-4159.1990.tb01968.x
被引量:
年份:
1990













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