A short amino acid sequence able to specify nuclear location.
摘要：
A short sequence of amino acids including Lys-128 is required for the normal nuclear accumulation of wild-type and deleted forms of SV40 large T antigen. A cytoplasmic large T mutant that lacks sequences from around Lys-128 localizes to the nucleus if the missing sequence is attached to its amino terminus. The implication that the sequence element around Lys-128 acts as an autonomous signal capable of specifying nuclear location was tested directly by transferring it to the amino termini of β-galactosidase and of pyruvate kinase, normally a cytoplasmic protein. Sequences that included the putative signal induced each of the fusion proteins to accumulate completely in the nucleus but had no discernible effect when Lys-128 was replaced by Thr. By reducing the size of the transposed sequence we conclude that Pro-Lys-Lys-Lys-Arg-Lys-Val can act as a nuclear location signal. The sequence may represent a prototype of similar sequences in other nuclear proteins.
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关键词：
Animals Cercopithecus aethiops Kidney Simian virus 40 Cell Line Cell Nucleus Chromosome Deletion DNA Restriction Enzymes beta-Galactosidase Viral Proteins
DOI：
10.1016/0092-8674(84)90457-4
被引量：
年份：
1984
Elsevier
Semantic Scholar
万方医学
dx.doi.org
Europe PMC
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