Differential localization of two myosins within nematode thick filaments

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作者：

DM Miller，I Ortiz，GC Berliner，HF Epstein

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摘要：

The body wall muscle cells of the nematode, Caenorhabditis elegans, contain two unique types of myosin heavy chain, A and B. We have utilized an immunochemical approach to define the structural location of these two myosins within body wall muscle thick filaments. By immunofluorescence microscopy, myosin B antibodies label the thick filament-containing A-bands of body wall muscle with the exception of a thin gap at the center of each A-band, and myosin A antibodies react to form a medial fluorescent stripe within each A-band. The complexes of these monoclonal antibodies with isolated thick filaments were negatively stained and studied by electron microscopy. The myosin B antibody reacts with the polar regions of all filaments but does not react with a central 0.9 μm zone. The myosin A antibody reacts with a central 1.8 μm zone in all filaments but does not react with the polar regions.

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关键词：

Animals Muscles Caenorhabditis Myosins Microscopy, Electron Antibodies, Monoclonal Fluorescent Antibody Technique Tissue Distribution