Bcl-G, a Novel Pro-apoptotic Member of the Bcl-2 Family
摘要：
A new member of the Bcl-2 family was identified, Bcl-G. The human gene consists of 6 exons, resides on chromosome 12p12, and encodes two proteins through alternative mRNA splicing, Bcl-G(long) and Bcl-G(short) consisting of 327 and 252 amino acids in length, respectively. Bcl-Gand Bcl-Ghave identical sequences for the first 226 amino acids but diverge thereafter. Among the Bcl-2 homology (BH) domains previously recognized in Bcl-2 family proteins, the BH3 domain is found in both Bcl-Gand Bcl-G, but only the longer Bcl-Gprotein possesses a BH2 domain. Bcl-GmRNA is expressed widely in adult human tissues, whereas Bcl-GmRNA was found only in testis. Overexpression of Bcl-Gor Bcl-Gin cells induced apoptosis although Bcl-Gwas far more potent than Bcl-G. Apoptosis induction by Bcl-Gdepended on the BH3 domain and was suppressed by coexpression of anti-apoptotic Bcl-Xprotein. Bcl-Xalso coimmunoprecipitated with Bcl-Gbut not with mutants of Bcl-Gin which the BH3 domain was deleted or mutated or with Bcl-G. Bcl-Gwas predominantly localized to cytosolic organelles, whereas Bcl-Gwas diffusely distributed throughout the cytosol. A mutant of Bcl-Gin which the BH2 domain was deleted displayed increased apoptotic activity and coimmunoprecipitated with Bcl-X, suggesting that the BH2 domain autorepresses Bcl-G.
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DOI：
10.1074/jbc.M005889200
被引量：
年份：
2001
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