The product of the human c-erbB-2 gene: a 185-kilodalton glycoprotein with tyrosine kinase activity
摘要：
Antibodies were raised against a synthetic peptide corresponding to 14 amino acid residues at the COOH-terminus of a protein deduced from the human c-erbB-2 nucleotide sequence. These antibodies immunoprecipitated a 185-kilodalton glycoprotein from MKN-7 adenocarcinoma cells. Incubation of the immunoprecipitates with (gamma-32P)ATP resulted in the phosphorylation of this protein on tyrosine residues. These results indicate that the human c-erbB-2 gene product is the 185-kilodalton glycoprotein that is associated with tyrosine kinase activity. Although the c-erbB-2 protein was predicted to encode a protein very similar to epidermal growth factor (EGF) receptor, EGF did not stimulate this kinase activity either in vivo or in vitro.
展开
关键词：
Hela Cells Humans Epidermal Growth Factor Receptor, Epidermal Growth Factor Glycoproteins Receptors, Cell Surface Amino Acid Sequence Phosphorylation Genes Oncogenes
DOI：
10.1126/science.3012781
被引量：
年份：
1986
Taylor & Francis
万方医学
Semantic Scholar
Europe PMC
NCBI
查看更多
Oxford Univ Press
JSTOR
NCBI
AACR
JBC
adsabs.harvard.edu
ResearchGate
ProQuest
EBSCO
jco.ascopubs.org
jimmunol.org
mendeley.com
jada.ada.org
scienceopen.com
mysciencework.com
SAGE
annclinlabsci.org
www.socolar.com
jleukbio.org
repository.sustech.edu
(全网免费下载)
science-open.com
rsfs.royalsocietypublishing.org
readcube.com
highbeam.com
erc.endocrinology-journals.org
clinchem.org
jpet.aspetjournals.org
nejm.org
scindeks.ceon.rs
sciencemag.org
cghjournal.org
theoncologist.alphamedpress.org
41.67.53.40
(全网免费下载)
onacademic.com
41.67.53.40
(全网免费下载)
repository.sustech.edu
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
