Purification of the human intestinal brush border membrane.
摘要：
A method is described to obtain brush border, i.e. microvillus membranes, from human intestinal epithelial cells. It is based on the sequential use of CaCl 2, differential centrifugation and Tris. The final preparation contains no DNA, no succinate dehydrogenase, traces only of (Na +K +)-ATPase, NADPH-cytochrome c reductase and β-glucuronidase and is characterized by a high specific activity in brush border membrane markers as well as its appearance on electron micrographs. The method may be applied to fresh or frozen intestine, to surgical specimens or peroral jejunal biopsies.
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关键词：
Cell Line, Tumor Vero Cells Animals Cercopithecus aethiops Mice Ferrous Compounds Indoles Vascular Endothelial Growth Factor Receptor-2 Crystallography, X-Ray Inhibitory Concentration 50
DOI：
10.1016/0005-2736(73)90434-3
被引量：
年份：
1973
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