Type-specific neutralization of the human immunodeficiency virus with antibodies to env-encoded synthetic peptides
摘要：
A synthetic peptide (SP-10-IIIB) with an amino acid sequence [Cys-Thr-Arg-Pro-Asn-Asn-Asn-Thr-Arg-Lys-Ser-Ile-Arg-Ile-Gln-Arg-Gly-Pro-Gl y -(Tyr); amino acids 303-321] from the human immunodeficiency virus (HIV) isolate human T-cell lymphotropic virus type III (HTLV-III) HTLV-IIIBenvelope glycoprotein gp120 was coupled to tetanus toxoid and used to raise goat antibodies to HIV gp120. Goat anti-SP- 10-IIIBserum bound to the surface of HTLV-IIIB-infected CEM T cells but not to the surface of HTLV-IIIRF-infected or uninfected CEM T cells. Anti-SP-10-IIIBantibodies also selectively bound to gp120 from lysates of HTLV-IIIBcells in immunoblot assays. Twenty-one percent of sera (28 of 175) from patients seropositive for HIV contained antibodies that reacted with SP-10-IIIBin RIA. Human anti-SP-10-IIIBantibodies affinity purified from acquired immunodeficiency syndrome (AIDS) patient serum bound to HTLV-IIIB-infected cells and immunoprecipitated gp120. Goat antibodies to SP-10-IIIBneutralized HTLV-IIIB(80% neutralization titer of 1/600), inhibited HTLV-IIIB-induced syncytium formation, but did not neutralize HIV isolates HTLV-IIIRFor HTLV-IIIMNor inhibit syncytium formation with these isolates. Also, goat antiserum to an homologous synthetic peptide [SP-10-IIIRF(A), (Cys)-Arg-Lys-Ser-Ile-Thr-Lys-Gly-Pro-Gly-Arg-Val-Ile-Tyr] from gp120 of HIV isolate HTLV-IIIRFinhibited syncytium formation by HTLV-IIIRF, but did not inhibit syncytium formation by HTLV-IIIBor by HTLV-IIIMN. Thus, the amino acid sequences of SP-10-IIIBand SP-10-IIIRF(A)define homologous regions of gp120 that are important in type-specific virus neutralization. The identification of these type-specific neutralizing epitopes should facilitate the design of a polyvalent, synthetic vaccine for AIDS.
展开
DOI：
10.1073/pnas.85.6.1932
被引量：
年份：
1988
Taylor & Francis
Semantic Scholar
掌桥科研
万方医学
dx.doi.org
查看更多
Europe PMC
(全网免费下载)
NCBI
JSTOR
NCBI
(全网免费下载)
Europe PMC
NCBI
adsabs.harvard.edu
ResearchGate
EBSCO
jimmunol.org
pubget.com
pnas.org
(全网免费下载)
scienceopen.com
deepdyve.com
The National Academy of Sciences of the United States of America
(全网免费下载)
core.ac.uk
mysciencework.com
mendeley.com
jvi.asm.org
onAcademic
pnas.org
biochemj.org
Europe PMC
(全网免费下载)
NCBI
(全网免费下载)
The National Academy of Sciences of the United States of America
(全网免费下载)
pnas.org
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
