The ligand-binding domain in metabotropic glutamate receptors is related to bacterial periplasmic binding proteins.

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作者：

O\"Hara, Patrick J.， Sheppard, Paul O.， Thógersen, Henning， Venezia, Domenick， Haldeman, Betty A.， McGrane, Vicki， Houamed, Khaled M.， Thomsen, Christian， Gilbert, Teresa L.， Mulvihill, Eileen R.

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摘要：

Receptors for the major excitatory neurotransmitter glutamate include metabotropic (G protein-coupled) and ionotropic (glutamate-gated ion channel) types. These receptors have large, presumably extracellular, amino-terminal domains. Sensitive sequence analysis techniques indicate that the metabotropic receptor extracellular domain is similar to bacterial periplasmic amino acid binding proteins. A structural model built using the observed similarity predicts a ligand-binding site, and mutants with conservative amino acid substitutions at this site are shown to have reduced ligand affinity. The metabotropic receptor extracellular domain is a member of a family of structural domains linked to a variety of receptor types, including ionotropic glutamate receptors.

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关键词：

Animals Bacterial Proteins Receptors, Glutamate Ligands Sequence Homology, Amino Acid Binding Sites Amino Acid Sequence Molecular Sequence Data Databases, Factual Carrier Proteins