The 5′-Terminal Nucleotide Sequences of the Double-Stranded RNA of Human Reovirus

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作者：

KI Miura，K Watanabe，M Sugiura，AJ Shatkin

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摘要：

The 5′-terminal nucleotide sequences of human reovirus double-stranded RNA were determined after labeling the RNA with [32P]phosphate by polynucleotide kinase. The 5′termini were labeled to only a limited extent prior to sequential oxidation, β -elimination, and phosphomonoesterase treatment, indicating that the terminal phosphates were in a modified, blocked configuration. Each genome segment, after removing the blocking group, contained the same two 5′-terminal sequences: GpApUp in one chain and G*pCp in the other. G*p is a derivative of guanylic acid, probably 2′-O-methyl-Gp, which renders the 5′-terminal sequence resistant to hydrolysis by alkali. The results indicate that the transcription of reovirus double-stranded RNA starts from the 3′end complementary to the G*pCp-terminal, resulting in the synthesis of single-stranded mRNA carrying the same 5′sequence as the G*pCp-chain. The presence of a modified nucleotide at the 5′terminus of the strand complementary to the mRNA template is a feature common to another double-stranded RNA virus, cytoplasmic polyhedrosis virus.

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关键词：

Biochemistry Modified 5′termini

DOI：

10.1073/pnas.71.10.3979

被引量：

139

年份：

1974