Ssn6-Tup1 is a general repressor of transcription in yeast.
摘要：
The homeodomain protein α2 and the SRF-like protein Mcm1 are required to establish cell type in the yeast Saccharomyces cerevisiae. Together, these regulatory proteins recognize a specific DNA operator, marking a set of genes for transcriptional repression. In this paper, we show that occupancy of the operator by α2-Mcm1 is not sufficient to bring about repression. Rather, repression is effected only when Ssn6 (a TPR protein) and Tup1 (a β-transducin repeat protein) are also present in the cell. We show that Ssn6 represses transcription when brought to a promoter by a bacterial DNA-binding domain and that Tup1 is required for this repression. Based on these and other results, we propose that Ssn6-Tup1 is a general repressor of transcription in yeast, recruited to target promoters by a variety of sequence-specific DNA-binding proteins.
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关键词：
Saccharomyces cerevisiae Repressor Proteins Immunoblotting Plasmids Transcription, Genetic Models, Genetic
DOI：
10.1016/0092-8674(92)90146-4
被引量：
年份：
1992
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