摘要：

Membranes and powders prepared from fluorinated polymers ( e.g. , Teflon™, PTFE) were investigated for their potential use as multifunctional supports for proteins, enzymes and sugars. To allow covalent coupling of biomolecules to PTFE, the surface of the material was treated with elementary sodium followed by oxidation with ozone or hydrogen peroxide. Derivatization steps were optimized in order to achieve highest loading and short reaction times. The enzymes alliinase (EC 4.4.1.4) and L-lactic dehydrogenase (EC 1.1.1.27), the lectin concanavalin A, and the sugar mannan were chosen as model substances. Highest alliinase loading was achieved at 0.08 µg×cm −2 .

展开