摘要：

The Pseudomonas sp. was immobilized on bamboo-carbon and the biodegradation of phenol by Pseudomonas sp. immobilized on bamboo-carbon was investigated. The effects of various parameters, such as the amount of immobilized biomass, pH and heavy metal ions, on phenol degradation were studied. Meanwhile, the relationship between the phenol concentration and reaction time were obtained, and the phenol degradation kinetics were studied. The advantage of microorganism immobilized on bamboo-carbon over activated sludge was analyzed by comparison experiments. The results show that phenol can be availably degraded by Pseudomonas sp. immobilized on bamboo-carbon. The degradation efficiency of phenol is the highest when the amount of immobilized biomass is 100 g·L-1 and the pH is within the range of 5 to 8. Heavy metal ions inhibit the degradation of phenol by immobilized Pseudomonas sp. with the order of inhibition degree of Ag+Cu2+Cd2+Co2+Cr6+Mn2+Zn2+. Compared with high initial phenol concentration, the degradation efficiency and degradation rate of low initial concentration phenol is higher and the time for the phenol to reach a steady concentration is shorter. The degradation process was found to involve two stage, both of which follow first order kinetics. Compared with activated sludge, the method of using Pseudomonas sp. immobilized on bamboo-carbon produces less sludge, separates microorganism from water more effectively, has a higher phenolremoval ability and capacity for degrading high concentration phenol, and the process of sludge recycling can be omitted.

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