Human CD4+ T lymphocytes with remarkable regulatory functions on dendritic cells and nickel-specific Th1 immune responses.

来自 EBSCO

阅读量：

作者：

Andrea Cavani，Francesca Nasorri，Caterina Prezzi，Silvia Sebastiani，Giampiero Girolomoni

展开

摘要：

The contribution of T helper (Th) and T cytotoxic (Tc) type 1 lymphocytes in the expression of to haptens has been amply documented. Conversely, the existence of T cell-based regulatory mechanisms has been poorly investigated. Here, we examined the properties of a subset of -specific + T cells displaying the cytokine profile (, , +/-, +/-) of T regulatory cells 1 () and with the potential to down-modulate to . clones were isolated from skin challenged with and peripheral blood of -allergic patients, and from the blood of healthy individuals. clones expressed CD25, , , , and the chain upon activation, whereas the antigen-3 was present on 50% of the clones. Monocytes precultured with cells in the presence of , or treated with -derived supernatant, exhibited a markedly diminished capacity to stimulate -specific responses. supernatants also blocked the differentiation of dendritic cells (DC) from monocytes, as well as DC maturation and IL-12 production induced by . As a consequence, the ability of DC to stimulate -specific and Tc1 responses was greatly impaired. These inhibitory effects were completely prevented by , but not , neutralization. In aggregate, the results indicate that cells can potently regulate the expression of -mediated allergic diseases via release of .

展开

关键词：

Humans Antigen-Presenting Cells Dendritic Cells CD4-Positive T-Lymphocytes Th1 Cells Dermatitis, Allergic Contact Flow Cytometry Cell Differentiation Antigen Presentation Adult