Enantiomeric separation of racemic clenbuterol by capillary zone electrophoresis
摘要:
A method for capillary electrophoretic enantiomeric separation of a racemic clenbuterol has been established with hydroxypropyl-β-cyclodextrin as the chiral selector. General equations and data analysis are presented to relate mobility to the equilibrium constants in simple binding equilibria and used to determine binding constants and thermodynamic parameters for host-guest complexation of clenbuterol enantiomers with hydroxypropyl-β-cyclodextrin as a selector. The effects of β-cyclodextrin type and concentration, buffer type, concentration and pH, as well as separation voltage and capillary temperature were investigated in detail. A maximal resolution of 6.78 was obtained. The binding constants of the host-guest complex of clenbuterol enantiomers with hydroxypropyl-β-cyclodextrin, K R-CD and K S-CD are 22.50 and 43.09 l mol -1 , respectively.
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关键词:
Electrophoretic enantiomeric separation Clenbuterol enantiomers Hydroxypropyl-β-cyclodextrin Binding constants Thermodynamic parameters
DOI:
10.1007/BF03246133
被引量:
年份:
2008
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