Cell surface recycling of internalized antigen permits dendritic cell priming of B cells.

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摘要：

Dendritic cells process internalized antigens to present degradative products on MHC for TCR recognition. Because antigen-exposed DCs also induce humoral immunity, DCs must also retain antigen in its native state for the engagement of BCR on B cells. Here, we demonstrate that antigen endocytosed by the inhibitory Fc receptor, FcγRIIB, accesses a nondegradative intracellular vesicular compartment that recycles to the cell surface, enabling interaction of native antigen with BCR on B cells. Immunization with IgG-opsonized, T independent antigens leads to enhanced humoral responses in a FcγRIIB and complement dependent manner. IC-loaded DCs trafficking to the splenic marginal zone can prime a T independent response in an FcγRIIB-dependent manner. Thus dendritic cells are equipped with both nondegradative and degradative antigen uptake pathways to facilitate antigen presentation to both B and T cells.

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关键词：

Animals Mice, Knockout Mice B-Lymphocytes Dendritic Cells T-Lymphocytes Cells, Cultured Antigens Lymphocyte Activation Cell Proliferation